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Microbiol. 50, 15 27 \(2003\):2542)/F 4/H/N/Rect[387.713 212.707 392.918 200.161]/Subtype/Link/Type/Annot>> endobj 469 0 obj <>/Subtype/Form/Type/XObject>>stream H0 endstream endobj 470 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 1 | Numerous mutational routes activating cellulose over-production underp\ in the WS phenotype. Previous studies of P. fluorescens identified spontaneous mutations in numerous DGC-encoding pathway\ s that generate the WS phenotype by s:2543)/F 4/H/N/Rect[422.971 212.707 427.434 200.161]/Subtype/Link/Type/Annot>> endobj 471 0 obj <>/Subtype/Form/Type/XObject>>stream H0 endstream endobj 472 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 28. McDonald, M. J., Gehrig, S. M., Meintjes, P. L., Zhang, X. X. & Rainey, P.\ B. Adaptive divergence in experimental populations of Pseudomonas fluorescens. IV. Genetic constraints guide evolutionary tra\ jectories in a parallel adaptive radi:2550)/F 4/H/N/Rect[434.121 170.828 439.326 158.161]/Subtype/Link/Type/Annot>> endobj 473 0 obj <>/Subtype/Form/Type/XObject>>stream H0 endstream endobj 474 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 26. Goymer, P. et al. Adaptive divergence in experimental populations of Pseu\ domonas fluorescens. II. Role of the GGDEF regulator WspR in evolution and development of the wrinkly spreader phenotype. 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Lind, P. A., Farr, A. D. & Rainey, P. B. Experimental evolution reveal\ s hidden diversity in evolutionary pathways. Elife 4, e07074 \(2015\).\r:2552)/F 4/H/N/Rect[136.156 506.706 141.361 494.16]/Subtype/Link/Type/Annot>> endobj 4 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 29. Lind, P. A., Farr, A. D. & Rainey, P. B. Experimental evolution revea\ ls hidden diversity in evolutionary pathways. Elife 4, e07074 \(2015\).\r:2553)/F 4/H/N/Rect[178.687 464.706 183.892 452.16]/Subtype/Link/Type/Annot>> endobj 5 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 29. Lind, P. A., Farr, A. D. & Rainey, P. B. Experimental evolution reve\ als hidden diversity in evolutionary pathways. Elife 4, e07074 \(2015\).\r:2554)/F 4/H/N/Rect[146.64 422.706 151.845 410.16]/Subtype/Link/Type/Annot>> endobj 6 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 29. Lind, P. A., Farr, A. D. & Rainey, P. B. Experimental evolution rev\ eals hidden diversity in evolutionary pathways. Elife 4, e07074 \(2015\).\r:2555)/F 4/H/N/Rect[241.392 401.827 246.445 389.16]/Subtype/Link/Type/Annot>> endobj 7 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 2 | Arrangement of pflu0184 and pflu0183 in the ancestral smooth gen\ otype and following gene fusion. a, The fusion mutation that occurred on four independent occasions \(underpinning FWS2, \ 39, 47 and 58\). The grey areas represe:2556)/F 4/H/N/Rect[152.776 380.827 161.062 368.16]/Subtype/Link/Type/Annot>> endobj 8 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 2 | Arrangement of pflu0184 and pflu0183 in the ancestral smooth ge\ notype and following gene fusion. a, The fusion mutation that occurred on four independent occasions \(underpinning FWS2,\ 39, 47 and 58\). The grey areas repres:2557)/F 4/H/N/Rect[172.347 359.706 181.275 347.16]/Subtype/Link/Type/Annot>> endobj 9 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 2 | Arrangement of pflu0184 and pflu0183 in the ancestral smooth g\ enotype and following gene fusion. a, The fusion mutation that occurred on four independent occasions \(underpinning FWS2\ , 39, 47 and 58\). The grey areas repre:2558)/F 4/H/N/Rect[170.949 338.706 179.608 326.16]/Subtype/Link/Type/Annot>> endobj 10 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 2 | Arrangement of pflu0184 and pflu0183 in the ancestral smooth \ genotype and following gene fusion. a, The fusion mutation that occurred on four independent occasions \(underpinning FWS\ 2, 39, 47 and 58\). The grey areas repr:2560)/F 4/H/N/Rect[224.329 223.327 232.875 210.66]/Subtype/Link/Type/Annot>> endobj 11 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 35. Romling, U., Galperin, M. Y. & Gomelsky, M. Cyclic di-GMP: the first 25 yea\ rs of a universal bacterial second messenger. Microbiol. Mol. Biol. Rev. 77, 1 52 \(2013\).\r:2561)/F 4/H/N/Rect[198.79 212.706 203.995 200.16]/Subtype/Link/Type/Annot>> endobj 12 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 36. Schirmer, T. & Jenal, U. Structural and mechanistic determinants of c-di-GM\ P signalling. Nat. Rev. Microbiol. 7, 724 735 \(2009\).\r:2562)/F 4/H/N/Rect[69.7586 202.327 74.9635 189.66]/Subtype/Link/Type/Annot>> endobj 13 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 37. Winsor, G. L. et al. Pseudomonas Genome Database: improved comparative anal\ ysis and population genomics capability for Pseudomonas genomes. Nucleic Acids Res. 39, D596 D600 \(2011\).\r:2563)/F 4/H/N/Rect[199.301 118.327 204.506 105.66]/Subtype/Link/Type/Annot>> endobj 14 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 38. Zhu, K., Choi, K. H., Schweizer, H. P., Rock, C. O. & Zhang, Y. M. Two aero\ bic pathways for the formation of unsaturated fatty acids in Pseudomonas aeruginosa. Mol. Microbiol. 60, 260 273 \(2006\).\ \r:2564)/F 4/H/N/Rect[149.567 97.3268 154.772 84.6602]/Subtype/Link/Type/Annot>> endobj 15 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 26. Goymer, P. et al. Adaptive divergence in experimental populations of Pse\ udomonas fluorescens. II. Role of the GGDEF regulator WspR in evolution and development of the wrinkly spreader phenotype. Gen\ etics 173, 515 526 \(2006\).\r:2565)/F 4/H/N/Rect[341.134 55.3268 346.339 42.6602]/Subtype/Link/Type/Annot>> endobj 16 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 28. McDonald, M. J., Gehrig, S. M., Meintjes, P. L., Zhang, X. X. & Rainey, P\ . B. Adaptive divergence in experimental populations of Pseudomonas fluorescens. IV. Genetic constraints guide evolutionary tr\ ajectories in a parallel adaptive rad:2566)/F 4/H/N/Rect[349.159 55.3268 354.363 42.6602]/Subtype/Link/Type/Annot>> endobj 17 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 26. Goymer, P. et al. Adaptive divergence in experimental populations of Pseud\ omonas fluorescens. II. Role of the GGDEF regulator WspR in evolution and development of the wrinkly spreader phenotype. Genet\ ics 173, 515 526 \(2006\).\r:2544)/F 4/H/N/Rect[211.189 610.494 216.287 600.254]/Subtype/Link/Type/Annot>> endobj 18 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 31. Spiers, A. J., Bohannon, J., Gehrig, S. M. & Rainey, P. B. Biofilm format\ ion at the air liquid interface by the Pseudomonas fluorescens SBW25 wrinkly spreader requires an acetylated form of cellulose\ . Mol. Microbiol. 50, 15 27 \(2003:2545)/F 4/H/N/Rect[217.392 610.494 221.487 600.254]/Subtype/Link/Type/Annot>> endobj 19 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 27. Bantinaki, E. et al. Adaptive divergence in experimental populations of Ps\ eudomonas fluorescens. III. Mutational origins of wrinkly spreader diversity. Genetics 176, 441 453 \(2007\).\r:2546)/F 4/H/N/Rect[105.783 589.494 110.267 579.254]/Subtype/Link/Type/Annot>> endobj 20 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 29. Lind, P. A., Farr, A. D. & Rainey, P. B. Experimental evolution reveals h\ idden diversity in evolutionary pathways. Elife 4, e07074 \(2015\).\r:2547)/F 4/H/N/Rect[112.268 589.494 117.263 579.254]/Subtype/Link/Type/Annot>> endobj 21 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 29. Lind, P. A., Farr, A. D. & Rainey, P. B. Experimental evolution reveals \ hidden diversity in evolutionary pathways. Elife 4, e07074 \(2015\).\r:2548)/F 4/H/N/Rect[64.2555 568.494 69.2506 558.254]/Subtype/Link/Type/Annot>> endobj 22 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 29. Lind, P. A., Farr, A. D. & Rainey, P. B. Experimental evolution reveals\ hidden diversity in evolutionary pathways. Elife 4, e07074 \(2015\).\r:2549)/F 4/H/N/Rect[208.509 557.994 213.653 547.754]/Subtype/Link/Type/Annot>> endobj 23 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 76. Krogh, A., Larsson, B., von Heijne, G. & Sonnhammer, E. L. L. Predicting tr\ ansmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. 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The structure function relationship of WspR, a Pseudo\ monas fluorescens response regulator with a GGDEF output domain. Microbiology 153, 980 994 \(2007\).\r:2567)/F 4/H/N/Rect[143.31 664.206 148.515 651.66]/Subtype/Link/Type/Annot>> endobj 43 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 2 | Arrangement of pflu0184 and pflu0183 in the ancestral smooth\ genotype and following gene fusion. a, The fusion mutation that occurred on four independent occasions \(underpinning FW\ S2, 39, 47 and 58\). The grey areas rep:2568)/F 4/H/N/Rect[237.57 569.706 246.759 557.16]/Subtype/Link/Type/Annot>> endobj 44 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 29. Lind, P. A., Farr, A. D. & Rainey, P. B. Experimental evolution re\ veals hidden diversity in evolutionary pathways. Elife 4, e07074 \(2015\).\r:2569)/F 4/H/N/Rect[104.765 380.827 109.97 368.16]/Subtype/Link/Type/Annot>> endobj 45 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 40. Mitra, A., Kesarwani, A. K., Pal, D. & Nagaraja, V. WebGeSTer DB-a transcri\ ption terminator database. Nucleic Acids Res. 39, D129 D135 \(2011\).\r:2570)/F 4/H/N/Rect[281.095 349.206 286.3 336.66]/Subtype/Link/Type/Annot>> endobj 46 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 3 | Transcription of fwsR from the fadA promoter is insufficient t\ o cause FWS. a, The construct PfadA fwsR, in which the promoter for fadA is fused to fwsR. b, The potential\ change in transcription caused by pr:2571)/F 4/H/N/Rect[242.702 275.827 251.248 263.16]/Subtype/Link/Type/Annot>> endobj 47 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 3 | Transcription of fwsR from the fadA promoter is insufficient \ to cause FWS. a, The construct PfadA fwsR, in which the promoter for fadA is fused to fwsR. b, The potentia\ l change in transcription caused by p:2572)/F 4/H/N/Rect[81.4578 223.206 90.6461 210.66]/Subtype/Link/Type/Annot>> endobj 48 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 29. Lind, P. A., Farr, A. D. & Rainey, P. B. Experimental evolution r\ eveals hidden diversity in evolutionary pathways. Elife 4, e07074 \(2015\).\r:2573)/F 4/H/N/Rect[150.076 55.2059 155.281 42.6602]/Subtype/Link/Type/Annot>> endobj 49 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 4 | The FWS phenotype requires translational fusion of the fadA and f\ wsR genes. a, Illustration of the construct fadA 3X fwsR. The fadA open reading frame was fused to that of fwsR an\ d is separated by three stop codons. :2574)/F 4/H/N/Rect[427.005 254.827 435.551 242.16]/Subtype/Link/Type/Annot>> endobj 50 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 4 | The FWS phenotype requires translational fusion of the fadA and \ fwsR genes. a, Illustration of the construct fadA 3X fwsR. 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O Connor, J. R., Kuwada, N. J., Huangyutitham, V., Wiggins, P. A. & Harwood\ , C. S. Surface sensing and lateral subcellular localization of WspA, the receptor in a chemosensory-like system leading to c-di\ -GMP production. Mol. Microbiol. 8:2581)/F 4/H/N/Rect[237.311 233.828 242.516 221.161]/Subtype/Link/Type/Annot>> endobj 68 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 5 | Fluorescence microscopy depicting the distribution of GFP-tagged\ proteins encoded from the fwsR locus. a f, Representative phase contrast images \(left\) and GFP fluorescence image\ s \(right\) demonstrating the subcellula:2582)/F 4/H/N/Rect[174.913 223.328 182.855 210.661]/Subtype/Link/Type/Annot>> endobj 69 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 28. McDonald, M. J., Gehrig, S. M., Meintjes, P. L., Zhang, X. X. & Rainey, \ P. B. Adaptive divergence in experimental populations of Pseudomonas fluorescens. IV. Genetic constraints guide evolutionary t\ rajectories in a parallel adaptive ra:2583)/F 4/H/N/Rect[284.312 65.8277 289.517 53.1611]/Subtype/Link/Type/Annot>> endobj 70 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 6 | Translational fusion of the transmembrane regions of mwsR to\ fwsR causes the FWS phenotype. a, The PfadA mwsR1218 fwsR construct. The first 1,218 bp of mwsR \(whic\ h encodes two transmembrane domain reg:2584)/F 4/H/N/Rect[204.449 55.2068 212.996 42.6611]/Subtype/Link/Type/Annot>> endobj 71 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd:Fig. 6 | Translational fusion of the transmembrane regions of mwsR to\ fwsR causes the FWS phenotype. a, The PfadA mwsR1218 fwsR construct. The first 1,218 bp of mwsR \(whi\ ch encodes two transmembrane domain re:2585)/F 4/H/N/Rect[462.385 401.707 471.574 389.161]/Subtype/Link/Type/Annot>> endobj 72 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 42. Sturtevant, A. The effects of unequal crossing over at the Bar locus in Dr\ osophila. Genetics 10, 117 147 \(1925\).\r:2587)/F 4/H/N/Rect[380.561 359.707 385.766 347.161]/Subtype/Link/Type/Annot>> endobj 73 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 44. Bridges, C. The bar gene  a duplication. Science 83, 210 211 \(1936\).\ \r:2588)/F 4/H/N/Rect[388.586 359.707 393.791 347.161]/Subtype/Link/Type/Annot>> endobj 74 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 3. Ohno, S. Evolution by Gene Duplication \(Springer, New York, 1970\).\r:2589)/F 4/H/N/Rect[406.993 349.207 409.595 336.661]/Subtype/Link/Type/Annot>> endobj 75 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 45. Lynch, M. & Conery, J. S. The evolutionary fate and consequences of duplica\ te genes. Science 290, 1151 1155 \(2000\).\r:2590)/F 4/H/N/Rect[410.831 349.207 416.036 336.661]/Subtype/Link/Type/Annot>> endobj 76 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 5. Long, M., Betran, E., Thornton, K. & Wang, W. The origin of new genes: glim\ pses from the young and old. Nat. Rev. Genet. 4, 865 875 \(2003\).\r:2591)/F 4/H/N/Rect[490.878 328.207 493.481 315.661]/Subtype/Link/Type/Annot>> endobj 77 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 12. Blount, Z. D., Barrick, J. E., Davidson, C. J. & Lenski, R. E. Genomic anal\ ysis of a key innovation in an experimental Escherichia coli population. Nature 489, 513 518 \(2012\).\r:2592)/F 4/H/N/Rect[490.728 307.207 495.933 294.661]/Subtype/Link/Type/Annot>> endobj 78 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 29. Lind, P. A., Farr, A. D. & Rainey, P. B. Experimental evolution \ reveals hidden diversity in evolutionary pathways. Elife 4, e07074 \(2015\).\r:2593)/F 4/H/N/Rect[497.169 307.207 502.374 294.661]/Subtype/Link/Type/Annot>> endobj 79 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 46. Thomson, T. M. et al. Fusion of the human gene for the polyubiquitination c\ oeffector UEV1 with Kua, a newly identified gene. Genome Res. 10, 1743 1756 \(2000\).\r:2594)/F 4/H/N/Rect[523.194 107.828 528.399 95.1611]/Subtype/Link/Type/Annot>> endobj 80 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 47. Marques, A. C., Vinckenbosh, N., Brawand, D. & Kaessmann, H. Functional div\ ersification of duplicate genes through subcellular adaptation of encoded proteins. 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Wang, X. J., Huang, Y., Lavrov, D. V. & Gu, X. Comparative study of human m\ itochondrial proteome reveals extensive protein subcellular relocalization after gene duplications. BMC Evol. Biol. 9, 275 \(\ 2009\).\r:2596)/F 4/H/N/Rect[263.588 380.707 268.793 368.161]/Subtype/Link/Type/Annot>> endobj 102 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 3. Ohno, S. Evolution by Gene Duplication \(Springer, New York, 1970\).\r:2597)/F 4/H/N/Rect[63.686 254.707 66.2885 242.161]/Subtype/Link/Type/Annot>> endobj 103 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 4. Bergthorsson, U., Andersson, D. I. & Roth, J. R. Ohno s dilemma: evolution \ of new genes under continuous selection. Proc. Natl Acad. Sci. USA 104, 17004 17009 \(2007\).\r:2598)/F 4/H/N/Rect[67.5247 254.707 70.1272 242.161]/Subtype/Link/Type/Annot>> endobj 104 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 45. Lynch, M. & Conery, J. S. 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Formation and longevity of chimer\ ic and duplicate genes in Drosophila melanogaster. Genetics 181, 313 322 \(2009\).\r:2602)/F 4/H/N/Rect[246.671 191.707 251.876 179.161]/Subtype/Link/Type/Annot>> endobj 108 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 50. Pawson, T. & Nash, P. Assembly of cell regulatory systems through protein i\ nteraction domains. Science 300, 445 452 \(2003\).\r:2603)/F 4/H/N/Rect[168.225 170.707 173.43 158.161]/Subtype/Link/Type/Annot>> endobj 109 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 27. Bantinaki, E. et al. Adaptive divergence in experimental populations of P\ seudomonas fluorescens. III. Mutational origins of wrinkly spreader diversity. Genetics 176, 441 453 \(2007\).\r:2604)/F 4/H/N/Rect[267.109 160.207 272.314 147.661]/Subtype/Link/Type/Annot>> endobj 110 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 39. Malone, J. G. et al. The structure function relationship of WspR, a Pseud\ omonas fluorescens response regulator with a GGDEF output domain. Microbiology 153, 980 994 \(2007\).\r:2605)/F 4/H/N/Rect[273.551 160.207 278.756 147.661]/Subtype/Link/Type/Annot>> endobj 111 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 41. O Connor, J. R., Kuwada, N. J., Huangyutitham, V., Wiggins, P. A. & Harwoo\ d, C. S. Surface sensing and lateral subcellular localization of WspA, the receptor in a chemosensory-like system leading to c-d\ i-GMP production. Mol. Microbiol. :2606)/F 4/H/N/Rect[279.992 160.207 285.197 147.661]/Subtype/Link/Type/Annot>> endobj 112 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 51. Huangyutitham, V., Guvener, Z. T. & Harwood, C. S. Subcellular clustering o\ f the phosphorylated WspR response regulator protein stimulates its diguanylate cyclase activity. mBio 4, e00242-13 \(2013\).\ \r:2607)/F 4/H/N/Rect[286.433 160.207 291.638 147.661]/Subtype/Link/Type/Annot>> endobj 113 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 52. Aldridge, P., Paul, R., Goymer, P., Rainey, P. & Jenal, U. Role of the GGDE\ F regulator PleD in polar development of Caulobacter crescentus. Mol. Microbiol. 47, 1695 1708 \(2003\).\r:2608)/F 4/H/N/Rect[78.6298 149.707 83.8347 137.161]/Subtype/Link/Type/Annot>> endobj 114 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 53. Paul, R. et al. Activation of the diguanylate cyclase PleD by phosphorylati\ on-mediated dimerization. J. Biol. Chem. 282, 29170 29177 \(2007\).\r:2609)/F 4/H/N/Rect[85.0709 149.707 90.2759 137.161]/Subtype/Link/Type/Annot>> endobj 115 0 obj <>/BS<>/Border[0 0 0]/Dest(SpringerNature_NATECOLEVOL_283.indd: 32. 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Site-di\ rected mutagenesis by overlap extension using the polymerase chain reaction. 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Farr Philippe Remigi Paul B. Rainey 10.1038/s41559-017-0283-7 http://dx.doi.org/10.1038/s41559-017-0283-7 journal Nature Ecology & Evolution © 2017, The Author(s) 2397-334X 10.1038/s41559-017-0283-7 springer.com springerlink.com 2010-04-23 True 10.1038/s41559-017-0283-7 springer.com springerlink.com Springer 2017-09-15T19:25:01+05:30 2017-09-15T19:24:14+05:30 2017-09-15T19:25:01+05:30 True VoR uuid:d01bb327-af9f-4b3e-b5c7-8b945fef7a82 uuid:a683bd0f-45d2-40b5-88f0-cfe4aa3f5931 default 1 converted uuid:e6929ab6-d4f8-4ed1-82fe-308046af1876 converted to PDF/A-2b pdfToolbox 2017-09-15T19:24:59+05:30 2 B Andrew D. 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